7OKW

1.62A X-ray crystal structure of the conserved C-terminal (CCT) of human OSR1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-07
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97950
Spacegroup name	C 1 2 1
Unit cell lengths	70.168, 49.424, 56.439
Unit cell angles	90.00, 99.44, 90.00

Refinement procedure

Resolution	34.610 - 1.620
R-factor	0.1856
Rwork	0.184
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v3s
RMSD bond length	0.006
RMSD bond angle	0.876
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.674	1.764
High resolution limit [Å]	1.620	1.623
Rmerge	0.114
Rmeas	0.123
Rpim	0.047
Number of reflections	19062	954
<I/σ(I)>	10
Completeness [%]	78.6	17.9
Redundancy	6.9
CC(1/2)	0.999	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		278	Morpheus A8: 0.06 M Divalents 0.1 M Buffer System 2 7.5 37.5 % v/v Precipitant Mix 4. Divalents:Mg chloride; Ca chloride. Buffer: pH 7.5 Sodium HEPES; MOPS (acid). Precipitants: 25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350.