7OJR
Bacillus subtilis phosphoglucomutase GlmM (phosphate bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 134.876, 134.876, 69.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.290 - 3.050 |
| R-factor | 0.2057 |
| Rwork | 0.203 |
| R-free | 0.25410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3PDK (B. anthracis GlmM structure) |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.290 | 3.260 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rpim | 0.130 | 2.580 |
| Number of reflections | 14081 | 2497 |
| <I/σ(I)> | 6.9 | 2.4 |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 19.6 | 17.3 |
| CC(1/2) | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298.15 | 0.1 M buffer system 1 (Imidazole; MES, pH 6.5), 0.09 M NPS (NaN03; Na2HPO4; (NH4)2SO4) and 37.5% MPD_P1K_P3350 (75% MPD, PEG 1K, PEG 3350) |
