7OJC
SaFtsZ(Q48A) complexed with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 71.804, 50.951, 88.232 |
Unit cell angles | 90.00, 110.99, 90.00 |
Refinement procedure
Resolution | 40.560 - 1.950 |
R-factor | 0.176 |
Rwork | 0.174 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rvn |
RMSD bond length | 0.008 |
RMSD bond angle | 0.895 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.560 | 2.020 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 21687 | 2151 |
<I/σ(I)> | 9.19 | 1.78 |
Completeness [%] | 98.5 | |
Redundancy | 3.2 | |
CC(1/2) | 0.994 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulphate, 10% (v/v) ethylene glycol, 0.1 M Tris-HCl pH 8.6, and 26% polyethylene glycol 5000 monomethyl ether |