7OIQ
Crystal structure of AP2 Mu2 in complex with FCHO2 WxxPhi motif (C2 crystal form)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.888, 64.539, 108.439 |
| Unit cell angles | 90.00, 111.95, 90.00 |
Refinement procedure
| Resolution | 55.700 - 1.850 |
| Rwork | 0.186 |
| R-free | 0.20870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Phaser |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.658 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.700 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.091 | 1.064 |
| Rmeas | 0.099 | 1.221 |
| Rpim | 0.039 | 0.577 |
| Number of reflections | 58302 | 1599 |
| <I/σ(I)> | 9.7 | 1.1 |
| Completeness [%] | 89.4 | 49.1 |
| Redundancy | 6.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 30mM Magnesium chloride hexahydrate, 30mM Calcium chloride dihydrate, 100mM Sodium HEPES MOPS (acid) pH 7.5, 20% v/v Ethylene glycol; 10 % w/v PEG 8000 |
