7OI1

Crystal structure of Synechocystis sp PCC6803 guanidinium hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2020-12-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	102.360, 140.210, 86.850
Unit cell angles	90.00, 119.75, 90.00

Refinement procedure

Resolution	29.150 - 1.900
R-factor	0.1739
Rwork	0.173
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3nio
RMSD bond length	0.007
RMSD bond angle	0.906
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.150	1.920
High resolution limit [Å]	1.900	1.900
Number of reflections	82121	2561
<I/σ(I)>	8.3
Completeness [%]	97.8
Redundancy	7
CC(1/2)	0.998	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1mM sodium citrate pH 5.5, 40% [v/v] PEG 600