7OHI

FCHO1-peptide-AP2 alpha ear complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-05-20
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97950
Spacegroup name	C 2 2 21
Unit cell lengths	61.168, 145.487, 88.808
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.400 - 1.410
R-factor	0.1975
Rwork	0.197
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b9k
RMSD bond length	0.006
RMSD bond angle	0.905
Data reduction software	DIALS (2.2.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.600	1.430
High resolution limit [Å]	1.410	1.410
Rmerge	0.059	2.810
Rmeas	0.062	3.054
Rpim	0.017	1.157
Number of reflections	73320	2558
<I/σ(I)>	17	0.2
Completeness [%]	95.9	63.6
Redundancy	11.9	6.4
CC(1/2)	1.000	0.253

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	1.6M Magnesium sulfate heptahydrate 0.1M MES pH 6.5. The crystal were subsequently cryo-protected with ~20% glycerol final.