7OHD
CRYSTAL STRUCTURE OF FERRIC MURINE NEUROGLOBIN CDLESS MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979499 |
| Spacegroup name | P 63 |
| Unit cell lengths | 95.941, 95.941, 58.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.020 - 1.800 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h6i |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.557 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.020 | 1.930 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 47796 | 7268 |
| <I/σ(I)> | 13.9 | 1 |
| Completeness [%] | 85.3 | |
| Redundancy | 12.05 | |
| CC(1/2) | 1.000 | 0.533 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.8 M ammonium sulfate, 0.1 M Bis-Tris pH 6.0, 3% isopropanol |
