7OH8

R17A mutant of Hfq protein from Neisseria meningitidis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-19
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 2
Unit cell lengths	61.683, 106.252, 27.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.860 - 1.700
R-factor	0.2432
Rwork	0.240
R-free	0.29530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pn0
RMSD bond length	0.008
RMSD bond angle	1.475
Data reduction software	XDS (20210323)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.250	40.250	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.245	0.048	2.040
Rmeas	0.255	0.051	2.128
Rpim	0.071	0.015	0.596
Total number of observations	268092	1886	13244
Number of reflections	21006	186	1077
<I/σ(I)>	9.4	32	1.4
Completeness [%]	99.8	99.2	96.1
Redundancy	12.8	10.1	12.3
CC(1/2)	0.997	0.999	0.566

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	293	PEG 1500, SPG pH 4