Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000029 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 89.838, 144.501, 168.432 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.657 - 2.700 |
| Rwork | 0.353 |
| R-free | 0.37080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ixo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.366 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.170 | 2.770 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.316 | 1.965 |
| Rmeas | 0.350 | 2.164 |
| Rpim | 0.149 | 0.900 |
| Number of reflections | 60957 | 4464 |
| <I/σ(I)> | 5.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 10.3 | 10.9 |
| CC(1/2) | 0.997 | 0.821 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.2 M magnesium chloride 0.1 M HEPES pH 7.5 25 % w/v PEG 3350 |
