Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-21 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000029 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 93.962, 169.894, 143.547 |
| Unit cell angles | 90.00, 96.74, 90.00 |
Refinement procedure
| Resolution | 49.095 - 2.872 |
| Rwork | 0.238 |
| R-free | 0.26220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ixo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.308 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.095 | 49.090 | 2.920 |
| High resolution limit [Å] | 2.870 | 15.730 | 2.870 |
| Rmerge | 0.211 | 0.028 | 1.684 |
| Rmeas | 0.250 | 0.033 | 1.995 |
| Rpim | 0.132 | 0.017 | 1.061 |
| Number of reflections | 101500 | 628 | 4649 |
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 99.6 | ||
| Redundancy | 6.9 | 6.5 | 6.7 |
| CC(1/2) | 0.996 | 1.000 | 0.540 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.2 M K/Na tartrate 20% w/v PEG 3350 |
