7OGQ

Plant peptide hormone receptor H1I2S1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-21
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.254716
Spacegroup name	P 2 21 21
Unit cell lengths	86.817, 88.078, 166.367
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.973 - 2.200
Rwork	0.198
R-free	0.23430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ixo
RMSD bond length	0.009
RMSD bond angle	1.452
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.973	46.930	2.250
High resolution limit [Å]	2.200	10.080	2.200
Rmerge	0.114	0.033	1.992
Rmeas	0.124	0.036	2.202
Rpim	0.047	0.013	0.930
Number of reflections	65575	793	4574
<I/σ(I)>	14.5
Completeness [%]	100.0
Redundancy	12.9	12.1	10.7
CC(1/2)	0.999	1.000	0.465

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291	0.2 M ammonium sulphate 0.1 M Bis-Tris 25 % w/v PEG 3350