7OGG
Nse5/6 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-18 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.116, 147.368, 74.444 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.850 - 3.290 |
| R-factor | 0.2989 |
| Rwork | 0.297 |
| R-free | 0.31670 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.771 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | HKL2Map |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.850 | 3.410 |
| High resolution limit [Å] | 3.290 | 3.293 |
| Rmerge | 0.075 | |
| Number of reflections | 16988 | 1639 |
| <I/σ(I)> | 16.77 | |
| Completeness [%] | 99.1 | |
| Redundancy | 5.4 | |
| CC(1/2) | 0.999 | 0.858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 292 | 2% (w/v) PEG 3350, 8% (v/v) 0.3 M Sodium malonate pH 7.5 |
