7OGE
GTPase HRAS mutant D33K under 200 MPa pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2016-07-23 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.374 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 39.568, 39.568, 160.645 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.177 |
| Rwork | 0.173 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 5P21 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.775 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.548 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.705 | |
| Number of reflections | 8578 | 1235 |
| <I/σ(I)> | 8.4 | 0.7 |
| Completeness [%] | 95.2 | 96 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 298 | 40 mM Tris HCL, 10 mM MgCl2, 2 mM DTE, 26-30 % PEG-400 |
