7OG7
Crystal structure of the copper chaperone NosL from Shewanella denitrificans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.28096 |
Spacegroup name | F 41 3 2 |
Unit cell lengths | 179.066, 179.066, 179.066 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.767 - 1.850 |
R-factor | 0.1954 |
Rwork | 0.194 |
R-free | 0.22160 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.770 | 1.890 |
High resolution limit [Å] | 1.850 | 1.850 |
Number of reflections | 21586 | 1322 |
<I/σ(I)> | 35.3 | |
Completeness [%] | 100.0 | |
Redundancy | 76.6 | |
CC(1/2) | 1.000 | 0.431 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES buffer at pH 7.5 and 1.4 M trisodium citrate tribasic dihydrate |