7OG7

Crystal structure of the copper chaperone NosL from Shewanella denitrificans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28096
Spacegroup name	F 41 3 2
Unit cell lengths	179.066, 179.066, 179.066
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.767 - 1.850
R-factor	0.1954
Rwork	0.194
R-free	0.22160
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.770	1.890
High resolution limit [Å]	1.850	1.850
Number of reflections	21586	1322
<I/σ(I)>	35.3
Completeness [%]	100.0
Redundancy	76.6
CC(1/2)	1.000	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES buffer at pH 7.5 and 1.4 M trisodium citrate tribasic dihydrate