7OFZ

Nontypeable Haemophillus influenzae SapA in complex with double stranded RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9200
Spacegroup name	P 4 21 2
Unit cell lengths	144.400, 144.400, 62.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	72.200 - 2.620
R-factor	0.219
Rwork	0.217
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m8u
RMSD bond length	0.010
RMSD bond angle	1.150
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.200	72.200	2.690
High resolution limit [Å]	2.620	11.720	2.620
Rmerge	0.112	0.023	1.571
Rmeas	0.129	0.027	1.804
Total number of observations	145122
Number of reflections	19346	432	2700
<I/σ(I)>	13.08	52.7	0.74
Completeness [%]	95.4	97.7	97.1
Redundancy	7.5	4.123	4.026
CC(1/2)	0.998	1.000	0.355

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	22% (w/v) PEG 3350, 0.25 M NaBr, 0.1 M Bis-Tris Propane pH 7.5