7OEX
Crystal structure of RBBP9 in complex with phenylalanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 130 |
| Detector technology | PIXEL |
| Collection date | 2020-02-03 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.956, 130.127, 38.993 |
| Unit cell angles | 90.00, 115.24, 90.00 |
Refinement procedure
| Resolution | 29.730 - 1.510 |
| R-factor | 0.1627 |
| Rwork | 0.161 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qs9 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.433 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.750 | 29.730 | 1.540 |
| High resolution limit [Å] | 1.510 | 8.290 | 1.510 |
| Rmerge | 0.068 | 0.034 | 0.669 |
| Rmeas | 0.073 | 0.036 | 0.742 |
| Rpim | 0.028 | 0.013 | 0.312 |
| Total number of observations | 2232 | 9392 | |
| Number of reflections | 49673 | 320 | 1841 |
| <I/σ(I)> | 14 | 42.2 | 2.3 |
| Completeness [%] | 96.1 | 96.3 | 72 |
| Redundancy | 7 | 7 | 5.1 |
| CC(1/2) | 0.999 | 0.999 | 0.813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 30% w/v PEG 4K, 0.1 M MES sodium salt pH 6.5 |
