7ODX

Cyanophage S-2L Succinoaminodeoxyadenylate synthetase (PurZ) bound to dGMP and dATP as an energy donor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980130
Spacegroup name	P 62 2 2
Unit cell lengths	108.180, 108.180, 142.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.495 - 1.696
R-factor	0.160215360219
Rwork	0.159
R-free	0.17601
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6fm1
RMSD bond length	0.010
RMSD bond angle	1.004
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.500	1.740
High resolution limit [Å]	1.696	1.700
Rmerge	0.081	2.661
Rmeas	0.082	2.695
Rpim	0.013	0.419
Number of reflections	54836	3903
<I/σ(I)>	34.1	1.7
Completeness [%]	99.8	98
Redundancy	39.6	39.4
CC(1/2)	1.000	0.694

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277.15	15% v/v Tacsimate; 2% w/v PEG 3350; 100 mM HEPES pH 7