Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-09-07 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.332, 145.878, 169.539 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 110.580 - 2.310 |
| Rwork | 0.255 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ixo |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.250 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 110.604 | 110.580 | 2.350 |
| High resolution limit [Å] | 2.310 | 12.650 | 2.310 |
| Rmerge | 0.319 | 0.070 | 3.518 |
| Rmeas | 0.332 | 0.072 | 3.652 |
| Rpim | 0.090 | 0.020 | 0.981 |
| Number of reflections | 106364 | 771 | 5146 |
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 100.0 | ||
| Redundancy | 26 | 22.5 | 26.7 |
| CC(1/2) | 0.998 | 0.998 | 0.703 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.2 M K/Na tartrate 20% w/v PEG 3350 |
