7ODU

Natural killer cell receptor NKR-P1B from Rattus norvegicus in complex with its cognate ligand Clr-11

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 32 2 1
Unit cell lengths	111.593, 111.593, 110.107
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.770 - 3.000
R-factor	0.2081
Rwork	0.208
R-free	0.25943
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ff8
RMSD bond length	0.008
RMSD bond angle	1.735
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	BALBES
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.770	49.770	3.180
High resolution limit [Å]	3.000	9.000	3.000
Rmerge	0.123	0.048	1.677
Rmeas	0.131	0.052	1.783
Rpim	0.045	0.019	0.602
Number of reflections	16302	677	2603
<I/σ(I)>	10.6	34.2	1.3
Completeness [%]	100.0	99.6	100
Redundancy	8.4	7.5	8.7
CC(1/2)	0.999	0.999	0.737

Crystallization Conditions

crystal ID	method	pH	temperature	details
2