Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.000033 |
| Spacegroup name | P 1 |
| Unit cell lengths | 77.539, 84.001, 89.061 |
| Unit cell angles | 99.18, 113.73, 108.41 |
Refinement procedure
| Resolution | 47.080 - 1.830 |
| Rwork | 0.204 |
| R-free | 0.21640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ixo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.084 | 47.080 | 1.860 |
| High resolution limit [Å] | 1.830 | 10.020 | 1.830 |
| Rmerge | 0.042 | 0.013 | 0.590 |
| Rmeas | 0.060 | 0.019 | 0.834 |
| Rpim | 0.042 | 0.013 | 0.590 |
| Number of reflections | 158310 | 966 | 7695 |
| <I/σ(I)> | 10.3 | ||
| Completeness [%] | 97.4 | ||
| Redundancy | 3.5 | 3.5 | 3.6 |
| CC(1/2) | 0.999 | 1.000 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 0.2 M ammonium sulfate 0.1 M citric acid pH 4.0 30% w/v PEG 1000 |
