7ODI
Crystal structure of bovine Hsc70(aa1-554)E213A/D214A in complex with methanesulfonamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.847, 49.566, 86.716 |
| Unit cell angles | 90.00, 99.84, 90.00 |
Refinement procedure
| Resolution | 42.870 - 1.830 |
| R-factor | 0.2307 |
| Rwork | 0.230 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fl9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.544 |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.900 | 1.930 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmeas | 0.065 | |
| Number of reflections | 48499 | 7625 |
| <I/σ(I)> | 11.87 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.4 | |
| CC(1/2) | 0.999 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 291 | PEG 8000, TMAO, HEPES-NaOH, glycerol |
