7ODH
Crystal structure of the O2-tolerant MBH-P242C from Ralstonia eutropha in its as-isolated state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.606, 95.676, 120.824 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.590 - 1.340 |
| R-factor | 0.1133 |
| Rwork | 0.112 |
| R-free | 0.13270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iub |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.245 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.590 | 1.390 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Number of reflections | 190645 | 27250 |
| <I/σ(I)> | 13.1 | |
| Completeness [%] | 99.7 | |
| Redundancy | 6.3 | |
| CC(1/2) | 1.000 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25% PEG 3350 5.5-6.5 pH 0.1M Bis-Tris |
