7ODD

Crystal structure of bovine Hsc70(aa1-554)E213A/D214A in complex with tricine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.918400
Spacegroup name	P 1 21 1
Unit cell lengths	66.057, 49.073, 87.271
Unit cell angles	90.00, 99.69, 90.00

Refinement procedure

Resolution	42.630 - 1.984
R-factor	0.2262
Rwork	0.225
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fl9
RMSD bond length	0.012
RMSD bond angle	1.569
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	2.100
High resolution limit [Å]	1.980	1.980
Rmeas	0.099
Number of reflections	37852	5867
<I/σ(I)>	8.57	8.57
Completeness [%]	98.2
Redundancy	3.4
CC(1/2)	0.998	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291	PEG 8000, TMAO, HEPES-NaOH, glycerol