7OD5

F(M197)H mutant structure of Photosynthetic Reaction Center From Rhodobacter Sphaeroides strain RV LSP crystallization

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0332
Spacegroup name	P 42 21 2
Unit cell lengths	99.984, 99.984, 238.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.090 - 2.100
R-factor	0.1805
Rwork	0.179
R-free	0.22070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6z1j
RMSD bond length	0.007
RMSD bond angle	0.903
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (1.18_3861)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.230
High resolution limit [Å]	2.100	2.100
Rmerge	0.120	1.360
Number of reflections	71237	11313
<I/σ(I)>	16.93	1.79
Completeness [%]	99.9
Redundancy	13.2
CC(1/2)	0.990	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	1-mono-oleyl-rac-glycerol (MO), 1,2,3-heptanetriol, Jeffamine M600, N,N-dimethyldodecylamine-N-oxide (LDAO)