7OCZ

Crystal Structure of the PID-3 RRM domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.28
Spacegroup name	P 65
Unit cell lengths	43.294, 43.294, 158.181
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.480 - 1.820
R-factor	0.1838
Rwork	0.182
R-free	0.20980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ocx
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1-4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.490	1.885
High resolution limit [Å]	1.820	1.820
Number of reflections	14314	1050
<I/σ(I)>	38.78	0.56
Completeness [%]	95.0
Redundancy	17.9
CC(1/2)	0.987	0.436

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.03 M Sodium nitrate, 0.03 M Sodium phosphatedibasic 0.03 M Ammonium sulfate 0.1 M HEPES, 0.1 M MOPS pH 7.5 20% v/v PEG 500 MME; 10% w/v PEG 20000