7OCM

K1K1H6, a potent recombinant minimal hepatocyte growth factor/scatter factor mimic

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	31.839, 59.648, 46.289
Unit cell angles	90.00, 103.85, 90.00

Refinement procedure

Resolution	35.890 - 1.700
R-factor	0.1653
Rwork	0.164
R-free	0.19450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bht
Data reduction software	iMOSFLM
Data scaling software	XSCALE (VERSION January 10, 2014 BUILT=20140115)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2-4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.940	44.940	1.300
High resolution limit [Å]	1.260	4.880	1.260
Rmerge	0.069	0.063	1.424
Rmeas	0.081	0.073	1.687
Rpim	0.041	0.036	0.890
Total number of observations	167746	3128	15200
Number of reflections	45262	820	4404
<I/σ(I)>	8.4	28.3	0.9
Completeness [%]	99.8	99.5	99.2
Redundancy	3.7	3.8	3.5
CC(1/2)	0.998	0.993	0.392

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	290.15	100 mM MOPS/HEPES pH 7.5, 30 mM sodium nitrate, 30 mM sodium phosphate dibasic, 30 mM ammonium sulphate, 20% v/v glycerol, 10% w/v PEG4000