7OCJ
Crystal structure of E.coli LexA in complex with nanobody NbSOS2(Nb14509)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-14 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.672, 112.672, 144.751 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.250 - 2.700 |
| Rwork | 0.228 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jhf |
| Data reduction software | XDS |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.790 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.126 | 0.730 |
| Number of reflections | 28568 | 8056 |
| <I/σ(I)> | 11.1 | 1.2 |
| Completeness [%] | 96.2 | |
| Redundancy | 7.4 | |
| CC(1/2) | 0.995 | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M lithium sulfate, 0.1 M sodium acetate pH 4.5, 30 % w/v PEG8000 |
