Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 112.896, 112.896, 101.669 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.700 - 2.300 |
| Rwork | 0.193 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tjs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.970 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.700 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.971 | |
| Number of reflections | 394340 | 1424 |
| <I/σ(I)> | 26 | |
| Completeness [%] | 100.0 | |
| Redundancy | 26 | |
| CC(1/2) | 0.069 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Morpheus screen followed by screening |
