7OBI
Consensus tetratricopeptide repeat protein type RV4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-03-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.912, 58.912, 189.517 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.020 - 3.000 |
| R-factor | 0.2278 |
| Rwork | 0.226 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hyz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.960 |
| Data reduction software | XDS (March 1, 2015) |
| Data scaling software | Aimless (0.5.4) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.020 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.066 | 1.739 |
| Rmeas | 0.071 | 1.871 |
| Rpim | 0.027 | 0.682 |
| Number of reflections | 8214 | 1143 |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 7 | 7.3 |
| CC(1/2) | 1.000 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 0.2 M magnesium chloride, 0.1 M sodium cacodylate pH 6.5, 50 % PEG 200 |
