7OB6

CPR-C4 - a conserved novel protease from the Candidate Phyla Radiation

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763, 1.2824
Spacegroup name	P 32 2 1
Unit cell lengths	123.392, 123.392, 96.530
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.791 - 2.598
Rwork	0.234
R-free	0.28530
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.418
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHELX
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.260	48.260	2.710
High resolution limit [Å]	2.598	9.000	2.600
Rmerge	0.094	0.018	4.086
Rmeas	0.106	0.020	4.649
Rpim	0.050	0.009	2.198
Number of reflections	26494	694	3143
<I/σ(I)>	14.4
Completeness [%]	99.8
Redundancy	8.5	7.8	8.4
CC(1/2)	1.000	1.000	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Morpheus screen (Molecular Dimensions)