7OAX
Crystal structure of the Chili RNA aptamer in complex with DMHBO+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.324, 100.352, 113.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.970 - 2.240 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.23730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7oa3 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.560 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.970 | 2.340 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.040 | 0.954 |
| Number of reflections | 25428 | 1271 |
| <I/σ(I)> | 19.3 | 1.9 |
| Completeness [%] | 94.5 | 91.5 |
| Redundancy | 6.6 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | PEG 400, MES, Spermine tetrahydrochloride |
