7OAS

Structural basis for targeted p97 remodeling by ASPL as prerequisite for p97 trimethylation by METTL21D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	58.295, 58.295, 142.851
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.221 - 1.819
R-factor	0.1994
Rwork	0.197
R-free	0.23820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lg1
RMSD bond length	0.007
RMSD bond angle	0.918
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.303	1.930
High resolution limit [Å]	1.819	1.820
Number of reflections	22897	3628
<I/σ(I)>	13.36
Completeness [%]	99.7
Redundancy	7.5
CC(1/2)	0.999	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	20 %v/v EtOH 0.1 M Tris 8.5 pH