7OAH
conserved hypothetical protein residues 311-335 from Candidatus Magnetomorum sp. HK-1 fused to GCN4 adaptors, mutant beta2/A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-10 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 26.126, 38.769, 132.277 |
| Unit cell angles | 90.00, 94.85, 90.00 |
Refinement procedure
| Resolution | 37.190 - 1.694 |
| Rwork | 0.220 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7oaa |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.008 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049 2013/06/30) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.190 | 1.800 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.082 | 0.912 |
| Number of reflections | 29121 | 4428 |
| <I/σ(I)> | 13.73 | 1.78 |
| Completeness [%] | 97.9 | 92.6 |
| Redundancy | 6.66 | 6.76 |
| CC(1/2) | 1.000 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1 M tri-sodium citrate pH 5.5, 20% (w/v) PEG 3000 |
