7OAD
conserved hypothetical protein residues 311-335 from Candidatus Magnetomorum sp. HK-1 fused to GCN4 adaptors, mutant beta1/A, crystal form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-06-15 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9998 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.351, 38.561, 99.254 |
| Unit cell angles | 90.00, 98.88, 90.00 |
Refinement procedure
| Resolution | 49.030 - 2.000 |
| Rwork | 0.254 |
| R-free | 0.29390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7oaa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049 2013/06/30) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.030 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.047 | 0.753 |
| Number of reflections | 18659 | 2993 |
| <I/σ(I)> | 19.67 | 2.44 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 6.71 | 6.63 |
| CC(1/2) | 1.000 | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 0.1M tri-sodium citrate pH 4.5, 7.1% PEG 10000 |
