Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-11-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9366 |
| Spacegroup name | P 31 |
| Unit cell lengths | 141.350, 141.350, 106.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.060 - 2.378 |
| R-factor | 0.2282 |
| Rwork | 0.227 |
| R-free | 0.24980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bvs |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.950 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | Aimless (0.2.17) |
| Phasing software | PHENIX (1.8.4) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.670 | 2.420 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Rmerge | 0.076 | 0.511 |
| Rmeas | 0.099 | 0.688 |
| Rpim | 0.063 | 0.456 |
| Number of reflections | 95144 | 4378 |
| <I/σ(I)> | 9.1 | 1.7 |
| Completeness [%] | 99.4 | 92.9 |
| Redundancy | 2.4 | 2 |
| CC(1/2) | 0.992 | 0.617 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | CRYSTALS GROWN UNDER OIL IN TERASAKI PLATES. DROPS FORMED FROM A 1:1 MIXTURE OF PROTEIN/DNA COMPLEX (10MG/ML COMPLEX, 20MM TRIS PH 7.5, 1MM EDTA, 0.1M NACL, 15% GLYCEROL) AND 1.4M NACITRATE, 0.1M TRIS PH 7.5., under oil |
