7O9S

Hantaan virus Gn in complex with Fab nnHTN-Gn2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	76.130, 76.900, 174.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.670 - 2.700
R-factor	0.2132
Rwork	0.211
R-free	0.26690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5opg 5drn
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX (1.19)
Refinement software	PHENIX (1.19)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	76.130	76.100	2.750
High resolution limit [Å]	2.700	7.330	2.700
Rmeas	0.084	0.039	0.759
Rpim	0.023	0.011	0.206
Total number of observations	377959
Number of reflections	28789	1599	1397
<I/σ(I)>	19.8	48.2	3.4
Completeness [%]	99.7	100	99.1
Redundancy	13.1	12	13.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.1 M sodium cacodylate pH 6.5, 5 % w/v PGA-LM and 20 % w/v PEG 3350