7O9Q

Crystal structure of the Awp1 (adhesin-like wall protein 1) A-domain from Candida glabrata

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97717
Spacegroup name	P 43 21 2
Unit cell lengths	83.280, 83.280, 274.240
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.810 - 1.850
R-factor	0.1875
Rwork	0.187
R-free	0.20690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Awp3bA
RMSD bond length	0.004
RMSD bond angle	0.674
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.810	1.920
High resolution limit [Å]	1.850	1.850
Number of reflections	83156	8101
<I/σ(I)>	12.64	1.62
Completeness [%]	99.2
Redundancy	2
CC(1/2)	0.998	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M MOPSO/Bis-Tris pH 6.5, 10% (w/v) PEG 8000, 20% 1,5-pentanediol, 0.5 mM erbium (III) chloride, 0.5 mM terbium (III) chloride, 0.5 mM ytterbium (III) chloride