7O9I
Escherichia coli Ffh in complex with pppGpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-13 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.976253 |
| Spacegroup name | P 41 |
| Unit cell lengths | 58.060, 58.060, 101.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.050 - 2.490 |
| R-factor | 0.2372 |
| Rwork | 0.235 |
| R-free | 0.27530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ng1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.271 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.050 | 2.580 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Number of reflections | 11747 | 1112 |
| <I/σ(I)> | 25.77 | |
| Completeness [%] | 99.6 | 96.29 |
| Redundancy | 19.2 | 14.4 |
| CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 293 | 0.2 M sodium chloride, 0.1 M CHES pH 9.5, 50% PEG 400 |
