7O9F

Bacillus subtilis Ffh in complex with ppGpp

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976254
Spacegroup name	P 41 21 2
Unit cell lengths	163.280, 163.280, 93.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.120 - 2.510
R-factor	0.2154
Rwork	0.213
R-free	0.25330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ng1
RMSD bond length	0.008
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.120	2.601
High resolution limit [Å]	2.510	2.510
Rmerge	0.138
Number of reflections	43600	4269
<I/σ(I)>	17.62
Completeness [%]	99.9
Redundancy	25.9
CC(1/2)	0.999	0.488

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9.5	293	0.1 M CHES pH 9.5, 30%(v/v) PEG 400