7O82

The L-arginine/agmatine antiporter from E. coli at 1.7 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	104.708, 175.592, 73.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.580 - 1.690
R-factor	0.195
Rwork	0.195
R-free	0.20310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j4i
Data reduction software	XDS (2018)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.810	1.790
High resolution limit [Å]	1.690	1.690
Rmeas	0.180
Rpim	0.020	0.700
Number of reflections	118568	5173
<I/σ(I)>	52.2	1.4
Completeness [%]	96.4
Redundancy	121.5
CC(1/2)	0.992	0.572

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	Tris-HCl pH 8.5, PEG400, NG