7O7D

Crystal structure of rsEGFP2 mutant V151A in the fluorescent on-state determined by synchrotron radiation at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 21 21 21
Unit cell lengths	50.990, 62.090, 69.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.280 - 1.400
R-factor	0.1535
Rwork	0.152
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dtx
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.280	1.440
High resolution limit [Å]	1.400	1.400
Rmeas	0.048	0.425
Number of reflections	42980	2669
<I/σ(I)>	17.26
Completeness [%]	97.4	96.7
Redundancy	3.9	3.9
CC(1/2)	0.999	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES pH 7.8 - 8.4 and 1.7 - 2.4 M ammonium sulfate