7O7A

Structure of the PL6 family alginate lyase Pedsa0632 from Pseudopedobacter saltans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.967700
Spacegroup name	P 1 21 1
Unit cell lengths	81.290, 47.290, 116.730
Unit cell angles	90.00, 98.03, 90.00

Refinement procedure

Resolution	57.790 - 1.990
R-factor	0.2119
Rwork	0.210
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6itg
RMSD bond length	0.008
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (29-NOV-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.490	2.040
High resolution limit [Å]	1.990	1.990
Number of reflections	58008	4451
<I/σ(I)>	11.7
Completeness [%]	94.7
Redundancy	6.9
CC(1/2)	0.998	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2 M K sulfate, 20% (w/v) PEG 3350