7O79

Structure of the PL6 family polysaccharide lyase Pedsa3628 from Pseudopedobacter saltans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.95372
Spacegroup name	P 1 21 1
Unit cell lengths	58.190, 51.746, 74.262
Unit cell angles	90.00, 97.22, 90.00

Refinement procedure

Resolution	48.490 - 1.930
R-factor	0.1789
Rwork	0.177
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6itg
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.490	2.050
High resolution limit [Å]	1.930	1.930
Number of reflections	32727	5026
<I/σ(I)>	8
Completeness [%]	98.5
Redundancy	6.6
CC(1/2)	0.996	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.2 M K phosphate, 20% (w/v) PEG 3350