7O77
Structure of the PL6 family alginate lyase Patl3640 from Pseudoalteromonas atlantica T6c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.525, 79.525, 269.472 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.160 - 2.321 |
| R-factor | 0.2517 |
| Rwork | 0.250 |
| R-free | 0.28230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gkd |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (29-NOV-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 2.460 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 42777 | 5845 |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 97.3 | |
| Redundancy | 24.8 | |
| CC(1/2) | 1.000 | 0.660 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 292 | 0.1 M Mg acetate, 0.1 M Na nitrate, 8% (w/v) PEG 10000, 0.15 mM CYMAL-7 |
