7O74
Structure of cyanase from Pseudomonas lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.146, 117.592, 106.991 |
| Unit cell angles | 90.00, 94.26, 90.00 |
Refinement procedure
| Resolution | 43.570 - 1.610 |
| R-factor | 0.1641 |
| Rwork | 0.164 |
| R-free | 0.18980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4y42 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | XSCALE (Feb 5, 2021) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.19) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.570 | 43.570 | 1.650 |
| High resolution limit [Å] | 1.610 | 7.190 | 1.610 |
| Rmerge | 0.169 | 0.055 | 2.706 |
| Rmeas | 0.183 | 0.060 | 2.931 |
| Total number of observations | 1407503 | ||
| Number of reflections | 202687 | 2383 | 14459 |
| <I/σ(I)> | 8.28 | 26.1 | 1.04 |
| Completeness [%] | 97.4 | 99 | 94.2 |
| Redundancy | 6.944 | 6.653 | 6.707 |
| CC(1/2) | 0.997 | 0.997 | 0.241 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 292.15 | MemGold B9 0.1M Potassium chloride 0.02M Tris7.0 20% w/v PEG 4000 |
