7O6N

Crystal structure of C. elegans ERH-2 PID-3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	44.987, 52.945, 125.608
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.790 - 2.170
R-factor	0.2107
Rwork	0.207
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7o6l
RMSD bond length	0.003
RMSD bond angle	0.652
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.790	2.248
High resolution limit [Å]	2.170	2.170
Number of reflections	16511	1463
<I/σ(I)>	13.3	1.3
Completeness [%]	98.9	90.04
Redundancy	12.8
CC(1/2)	0.998	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Magnesium formate dihydrate 20 % w/v PEG 3350