7O6L

Crystal structure of C. elegans ERH-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-05
Detector	DECTRIS PILATUS3 X 2M
Wavelength(s)	0.873
Spacegroup name	P 1 21 1
Unit cell lengths	38.267, 54.053, 46.762
Unit cell angles	90.00, 93.42, 90.00

Refinement procedure

Resolution	31.200 - 1.500
R-factor	0.2014
Rwork	0.201
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2nml
RMSD bond length	0.010
RMSD bond angle	1.042
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.350	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	30574	3039
<I/σ(I)>	9.99	1.14
Completeness [%]	99.8	99.47
Redundancy	7.3
CC(1/2)	1.000	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M Imidazole/MES pH 6.5, 0.03 M MgCl2, 0.03 M CaCl2, 20% (v/v) ethylene glycol, 10% (v/v) PEG 8000