7O6D
Crystal structure of human mitochondrial ferritin (hMTF) Fe(II)-loaded for 3 minutes under anaerobic environment
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-17 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.90001 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 184.090, 184.090, 184.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.090 - 1.470 |
R-factor | 0.1466 |
Rwork | 0.146 |
R-free | 0.15710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1r03 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.367 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.110 | 2.320 | 1.550 |
High resolution limit [Å] | 1.470 | 2.200 | 1.470 |
Rmerge | 0.108 | 0.227 | 0.671 |
Rmeas | 0.113 | 0.250 | 0.702 |
Rpim | 0.024 | 0.108 | 0.151 |
Number of reflections | 45819 | 1866 | 6565 |
<I/σ(I)> | 14.6 | 4.4 | 4.2 |
Completeness [%] | 100.0 | 93 | 100 |
Redundancy | 21.9 | 4.4 | 21.4 |
CC(1/2) | 0.999 | 0.944 | 0.932 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 | |
1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 |