7O63

High resolution crystal structure of human mitochondrial ferritin (hMTF)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97622
Spacegroup name	F 4 3 2
Unit cell lengths	184.133, 184.133, 184.133
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.100 - 1.160
R-factor	0.1258
Rwork	0.125
R-free	0.13970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1r03
RMSD bond length	0.008
RMSD bond angle	1.513
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.100	1.220
High resolution limit [Å]	1.160	1.160
Rmerge	0.094	0.713
Rmeas	0.098	0.760
Rpim	0.030	0.200
Number of reflections	92014	13215
<I/σ(I)>	15.5	3.7
Completeness [%]	99.9	99.9
Redundancy	20.2	14.4
CC(1/2)	0.999	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		281.15	1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0