7O63
High resolution crystal structure of human mitochondrial ferritin (hMTF)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97622 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.133, 184.133, 184.133 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.100 - 1.160 |
| R-factor | 0.1258 |
| Rwork | 0.125 |
| R-free | 0.13970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r03 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.513 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.100 | 1.220 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.094 | 0.713 |
| Rmeas | 0.098 | 0.760 |
| Rpim | 0.030 | 0.200 |
| Number of reflections | 92014 | 13215 |
| <I/σ(I)> | 15.5 | 3.7 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 20.2 | 14.4 |
| CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 281.15 | 1.6-2 M MgCl2 6H2O and 0.1 M bicine pH 9.0 |
