7O5L
Crystal structure of S-adenosyl-L-homocysteine hydrolase from Synechocystis sp. PCC 6803 cocrystallized with adenosine in the presence of Rb+ cations
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9184 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 120.770, 196.070, 82.052 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.630 - 1.740 |
R-factor | 0.1881 |
Rwork | 0.188 |
R-free | 0.21440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3onf |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.634 | 48.634 | 1.850 |
High resolution limit [Å] | 1.740 | 5.200 | 1.740 |
Rmerge | 0.123 | 0.047 | 1.433 |
Rmeas | 0.129 | 0.049 | 1.502 |
Total number of observations | 1224382 | ||
Number of reflections | 98599 | 3962 | 15150 |
<I/σ(I)> | 13.7 | 43.8 | 1.36 |
Completeness [%] | 99.2 | 99.6 | 95.3 |
Redundancy | 12.418 | 11.366 | 11.027 |
CC(1/2) | 0.999 | 0.999 | 0.687 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 292 | 0.2MSodium fluoride, 0.1MBis-Tris propane pH 7.5, 20% w/vPEG 3350 |